Sclerotium density, assessed based on colony forming units (CFU), ranged from 156 to 1,108/g root tissue. To quantify the sclerotia, the methodology of Cloud and Rupe (1991) and Mengistu et al. phaseolina to colonize dead substrates, senesced stem segments from the main plant species representing the agricultural system of southern Brazil were exposed on naturally infested soil for 30 and 60 days. phaseolina natural infection was also determined for the roots of weeds grown in the experimental area. ![]() The density of free sclerotia on the soil was determined for plots of crop rotation (soybean-corn) and soybean monoculture soon after soybean harvest. In field experiments, the density of Macrophomina phaseolina microsclerotia in root tissues of naturally colonized soybean cultivars was quantified.
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